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Methods for Fragments Screening Using Surface Plasmon Resonance
Engelsk Paperback
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Methods for Fragments Screening Using Surface Plasmon Resonance
Engelsk Paperback

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Om denne bog

This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.

Product detaljer
Sprog:
Engelsk
Sider:
113
ISBN-13:
9789811615382
Indbinding:
Paperback
Udgave:
ISBN-10:
9811615381
Kategori:
Udg. Dato:
17 sep 2022
Længde:
0mm
Bredde:
155mm
Højde:
235mm
Forlag:
Springer Verlag, Singapore
Oplagsdato:
17 sep 2022
Forfatter(e):
Kategori sammenhænge