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CRISPR-Cas Methods
Engelsk Paperback
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CRISPR-Cas Methods
Engelsk Paperback

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Om denne bog
This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
Product detaljer
Sprog:
Engelsk
Sider:
278
ISBN-13:
9781071606186
Indbinding:
Paperback
Udgave:
ISBN-10:
1071606182
Udg. Dato:
5 jul 2020
Længde:
0mm
Bredde:
178mm
Højde:
254mm
Forlag:
Springer-Verlag New York Inc.
Oplagsdato:
5 jul 2020
Forfatter(e):
Kategori sammenhænge