Biochemical methods are widely utilized in various branches of biological science, including agriculture. The biochemical aspect is a crucial part of plant physiology, and it is used to explain most of the physiological processes in plants and crops. Biochemical Aspects of Plant Physiology: Technology and Methodology is primarily intended for postgraduate students and researchers in universities and research institutes. Given its comprehensive coverage of both theoretical and practical aspects of the biochemical aspects of plant physiology, it is likely to be useful for any student enrolled in theoretical or practical plant physiology or biochemistry courses. This book is built on three foundational principles: first, it presents the theoretical principles and practical techniques with descriptions of various biochemical assays; second, it contains detailed experimental protocols to perform experiments, along with a collection and description of principles; and third, it provides practical knowledge regarding the techniques used to investigate the properties of macromolecules. Additionally, the book explains how to determine the charge of weak acids, bases, and macromolecules by considering their chemical environment and how to measure the macromolecular concentration of solutions by spectrophotometry. Furthermore, the book offers guidance on designing protocols for the purification of proteins from cell cultures or tissues. It is an excellent resource for conducting practical classes for undergraduate and postgraduate students in plant physiology, biochemistry, biotechnology, microbiology, agricultural science, environmental science, nutrition, pharmaceutical science, and other biology-related subjects. The books focus on the technologies and methods used in the biochemical basis of plant physiology, such as photosynthesis, photorespiration, plant pigments, carbon and nitrogen assimilation, plant nutrients, phenols, secondary metabolites, nucleic acids, and vitamins, will be of great use not only to postgraduate students but also to research workers.