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Non-Alcoholic Steatohepatitis

- Methods and Protocols
Engelsk Paperback

Non-Alcoholic Steatohepatitis

- Methods and Protocols
Engelsk Paperback
Tjek vores konkurrenters priser

This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; protocols for isolating hepatocytes and Kupffer cells, bone marrow derived macrophages, and adipocytes; techniques to develop human pluripotent stem cells-derived liver organoids; single-cell and RNA-sequencing; and a description of how to extract exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Thorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols is a valuable resource for new and experiences investigators studying NASH, and serves as an essential reference on NASH for basic and clinical researchers and students. 


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This volume contains a comprehensive collection of laboratory protocols used by researchers to analyze varied aspects of non-alcoholic steatohepatitis (NASH). The chapters in this book cover topics such as methods for histological diagnosis of NASH; the purpose of generating an in vivo NASH model; protocols for isolating hepatocytes and Kupffer cells, bone marrow derived macrophages, and adipocytes; techniques to develop human pluripotent stem cells-derived liver organoids; single-cell and RNA-sequencing; and a description of how to extract exosomes and exosomal miRNAs from mesenchymal stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Thorough and practical, Non-Alcoholic Steatohepatitis: Methods and Protocols is a valuable resource for new and experiences investigators studying NASH, and serves as an essential reference on NASH for basic and clinical researchers and students. 


Produktdetaljer
Sprog: Engelsk
Sider: 361
ISBN-13: 9781071621301
Indbinding: Paperback
Udgave:
ISBN-10: 1071621300
Kategori: Medicinsk genetik
Udg. Dato: 27 feb 2023
Længde: 25mm
Bredde: 253mm
Højde: 177mm
Forlag: Springer-Verlag New York Inc.
Oplagsdato: 27 feb 2023
Forfatter(e):
Forfatter(e)


Kategori Medicinsk genetik


ISBN-13 9781071621301


Sprog Engelsk


Indbinding Paperback


Sider 361


Udgave


Længde 25mm


Bredde 253mm


Højde 177mm


Udg. Dato 27 feb 2023


Oplagsdato 27 feb 2023


Forlag Springer-Verlag New York Inc.

Kategori sammenhænge